Laureane wrote:
>> Hi!!
>> We have some problems using the gap4 program..and one of them is:
> We have tried to find in the program manuel how to add a sequence to a
> database that already exists, without success!...We have already put
> several sequences together to make a contig, but we now would like to add
> one or more sequences to it. If someone knows how to do it...
>> Something else we would like to do is to add a sequence that has not a
> chromatograph but only the text..
>> THANK YOU for helping us!!
I have a method which works, but im not sure whether it is the
'officail' approach..
The way i do it is very similar to starting a contig database.
1- if necessary convert your sequence files into staden format.
2- create a list of file names using the unix ls and pipe commnads.
3- start gap4, and open up existing database.
4- go to assembly ands select 'normal shotgun assembly' and fill in
the blanks on the form.
5- click on okay and hopefully the sequences are added to the existing
database.
Does this answer your query??
Enjoy....
Adrian
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NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL
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Adrian Jenkins
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