Pregap and sequencing in both directions

Andy Law Andy.Law at bbsrc.ac.uk
Tue Dec 3 12:33:31 EST 1996

I'm just starting up with Staden, and I have a quick question. All our
clones are routinely sequenced in both directions. Do I need to run pregap
on them in 2 batches, specifying different offsets for the different
sequencing primers? I'm a little unclear as to how the direction is
determined in pregap as well. Should I specify one offset as being negative
(since it is the other side of the cloning site)?

Ta muchly in advance,

Andy Law
( Andy.Law at bbsrc.ac.uk )
( Big Nose in Edinburgh )

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