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Help w/ a first time sequence analysis

Fang Qingquan qfang at WAM.UMD.EDU
Wed Mar 15 10:23:46 EST 1995


	FTP your ABI sequence file (not the file with .seq !) to the
computer where STADEN is installed.  Open STADEN porgram, use the command:
"TED -ABI <seq-file-name>" to look the chematogram and to check
miss-calling base.  Then, save your sequence for further processing, for
example, using XDAP/XBAP to assembly contigs. 

 +==================================================+
 |   Qingquan Fang, Ph.D.                           |
 |   Postdoctoral Research Associate                |
 |   Center for Agricultural Biotechnology (CAB)    |
 |   2113 Agriculture/Life Sciences Surge Bldg.     |
 |   University of Maryland                         |
 |   College Park, MD 20742-3351                    |
 |        --------------------------------          |
 | Phone: (301)-405-6937       Fax: (301)-314-9075  |
 | Internet: qfang at wam.umd.edu / qfang at glue.umd.edu |
 +==================================================+

On 14 Mar 1995, Ned Lally wrote:

> We have recently purchased and installed the the Staden package.  After
> reading the manual several times over, we have a good understanding of the
> components of the program.  What we cannot seem to find after reading is
> how to take a single sequence file(s) from an ABI 373 move it into the the
> package and perform a simple manipulation. 
> 
> Can anybody provide us w/ help on this matter? 




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