sarita malik wrote:
>> i have a very simple Query
> how do u estimate protein concentration of a whole cell protein sample denatured in lammeli buffer for performing PAGE. i tried Lowrys method and Brad ford but both methods gave inconsistent results. when compared compared with electrophoresis of a known protein sample.
SDS interferes with the Bradford assay. It's been years since I've done
a Lowry, so I can't remember if it can tolerate it.
However, you can use the Pierce BCA assay, but not if you have reducing
agents in your sample.
nick_theodorakis at hotmail.com