i have a very simple Query
how do u estimate protein concentration of a whole cell protein sample denatured in lammeli buffer for performing PAGE. i tried Lowrys method and Brad ford but both methods gave inconsistent results. when compared compared with electrophoresis of a known protein sample.
C/o Dr. J.S. Virdi
Microbial Pathogenicity Laboratory
Dept. of Microbiology
Universitty of Delhi South Campus
Benito Juarez Road
New Delhi - 110 021