Chris B. <chris.b.nospam at nospam.interia.pl> wrote:
> "Dr Engelbert Buxbaum" wrote:
>> Unfortunately, pre-activated gels (either CNBr or other chemistries) are
> not cheap.
>> I know about CNBr pre-activated gels and their prices, that's why I was
> searching for something cheaper.
>> Chitosan + glutaraldehyde worked fine for me.
To ask a related question ... other than the CNBr activation method,
what are some alternatives? (that is, if I want to prepare
a DEAE-sepharose using DEAE and sepaharose - what are some methods
that will work??) (will not glutaraldehyde mess with protein structure
if you are using it to immobilize it onto a matrix like chitosan?)