On Wed, 19 Jan 2005 15:44:34 +0100, Gerd Hoffmann
<gerd.hoffmann at nambition.com> wrote:
>>we want to try to determine protein concentration in a solution
>containing fragments of "membrane-protein-crystalls". We
>want to try this with a bradford assay.
>Unfortunately these fragments are about 1 micron in diameter
>and therefore to big for a simple photometric determination.
>So we have to get the proteins out of the membrane. We can
>do this for example by using detergents. So here are my
>questions: Is there any inteference between the bradford assay
>and the detergent? And if so, are there other possibilities
>to measure the concentration of the protein in our solution?
The Bradford assay is not compatible with most detergents. You can try
the Pierce BCA assay, which works in detergent, but you can't have
reducing agents in it.
nick_theodorakis at hotmail.com