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Bradford Protein Assay in membrane crystals

Nick Theodorakis nick_theodorakis at hotmail.com
Wed Jan 19 21:34:32 EST 2005


On Wed, 19 Jan 2005 15:44:34 +0100, Gerd Hoffmann
<gerd.hoffmann at nambition.com> wrote:

>Hi,
>
>we want to try to determine protein concentration in a solution
>containing fragments of "membrane-protein-crystalls". We
>want to try this with a bradford assay.
>Unfortunately these fragments are about 1 micron in diameter
>and therefore to big for a simple photometric determination.
>So we have to get the proteins out of the membrane. We can
>do this for example by using detergents. So here are my
>questions: Is there any inteference between the bradford assay
>and the detergent? And if so, are there other possibilities
>to measure the concentration of the protein in our solution?
>

The Bradford assay is not compatible with most detergents. You can try
the Pierce BCA assay, which works in detergent, but you can't have
reducing agents in it.

Nick

-- 
Nick Theodorakis
nick_theodorakis at hotmail.com
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http://theodorakis.net/contact.html



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