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[Protein-analysis] trypsin digestion

nithya blueylife at gmail.com
Fri Dec 16 23:15:31 EST 2005


I have done a trypsin digestion of a coloured protein.........
my protocol is 1mg/ml trypsin in 0.1% acetic acid.......
protein soln 40 ug/ml in 100 mM AmBic buffer ph:8.5
overnight incubation
prot: trypsin ratio :  100:1/ 50:1

my boss says that i need to c a reduction in color of the prot if
digestion has occurred.....

is ther any other way to confirm that digestion has occurred
completely?

I would welcome any suggestions on the protocol as well

have to do MS of the digested peptides

help me

thanks

Nithya



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