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Help me! AIR BUBBLES trapped in HPLC column...

LIn Chung-Yen lin at formosa.g1.ntu.edu.tw
Tue Apr 1 04:54:10 EST 1997


Rainer Hofmann wrote:

  newera at plaza.snu.ac.kr () wrote:
  >Some or probably quite many air bubbles seem to be trapped in my
  HPLC column: 18C reverse phase column, pore size 7um.
  >Could you give me any suggestions to remedy this problem as quickly
  as possible?
  >I am much disappointed not to be able to proceed my experiments any
  more.

  I guess the problem is poor degassing of solvents. At pressure
  levels
  in a hplc column there aren't any free gas bubbles. But when
  pressure
  drops at the column's outlet gas is set free again and bubbles
  disturb
  detection.
  You should use Helium to purge solvents and to keep them under a
  Helium atmosphere. Online degassing systems are also available.

  --
  Rainer Hofmann
  rhofmann at freiburg.netsurf.de
  Kalkackerweg 20                                       fax: +49 761
  4764136
  79111 Freiburg                                      phone: +49 761
  4764135
  Germany

 Hi:
You can degas solvents under sonicator and use air pump to remove air
bubles in solvent.
Try it, it works to me many years!!

Lin Chung-Yen
                                                  Institute of  Zoology,
National Taiwan Univ.

Taiwan




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