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staining of phosphoproteins

Jens Krieger krieger at mibm1.ruf.uni-freiburg.de
Thu Feb 1 11:17:14 EST 1996

Im working on the major yolk proteins of two european freshwater teleosts.
One method is SDS-gelelektrophoresis. So I now that one of the major yolk 
proteins is a highly phosphorylated protein named phosvitin. To stain it 
on the acrylamidgels, I cnat use coomassie, because it wont stain 
phosphoproteins. So I use the method of Cutting and Roth (1973), staining 
them with methylgreen. But....I doesnt work anyway.

Is there anyone "outside" who will know a recipe for staining 
phosphoproteins on acrylamidgels, or could give me any hint?

Thanks in advance,
			Jens Krieger.

|  Jens Krieger		"Solange die Alpen noch stehen, |
|  TU Dresden, Zoology		wird es keine		|
|  Tel.:+49.351.463.5465  	   Gerechtigkeit geben."|
|							|
| krieger at rcs.urz.tu-dresden.de		H.Achternbusch	|
\ krieger at mibm.ruf.uni-freiburg.de			/

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