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[Plant-education] Photoperiodic flowering timelapse - teaching tool

Jon Monroe via plant-ed%40net.bio.net (by monroejd from jmu.edu)
Fri Jun 6 15:21:34 EST 2014

Hi all,

This is a forwarded message from the Arabidopsis BIOSCI newsgroup that 
may be of interest to Plant-ed community.

Jon Monroe
Plant-ed moderator

-------- Original Message --------
Subject: 	[Arabidopsis] Photoperiodic flowering timelapse - teaching tool
Date: 	Fri, 6 Jun 2014 08:54:22 -0700
From: 	Takato Imaizumi <takato from u.washington.edu>
To: 	<arab-gen from magpie.bio.indiana.edu>
CC: 	Takato Imaizumi <takato from uw.edu>

Dear Arabidopsis community:

We study the molecular mechanisms of photoperiodic flowering in Arabidopsis.
Recently we made timelapse movies showing how Arabidopsis plants respond to a certain day length.

This timelapse showed about 2 months of growth of a WT (Col-0) plant and constans (co) mutant grown under long-day conditions. Images were originally taken every 5 min during the day.
This video shows that photoperiodic information drastically changes the timing of flowering and plant biomass. It also shows leaf movement of a gigantic constans mutant and circumnutation of stems as well.

This type of movie is also helpful to show undergraduate students how dynamic and plastic plant development is.

The movie is very short and if you can use it for your classes and/or tell your friends and other instructors in your department about it, I would greatly appreciate it.

The  movie, 45 sec version.

The movie, 2 min version (higher time resolution version)

(Movie caption)
An Arabidopsis thaliana wild-type (Col-0) plant (left) and a constans (co-101) mutant (right) that cannot sense differences in day length have been grown under long-day conditions (16 hours light and 8 hours dark, 22°C).
Arabidopsis is a long-day plant, so this growth condition accelerates flowering for wild type plants, but not for the co mutant. The co mutant behaves like a wild-type plant grown in short-day conditions.

co-101: Takada S, and Goto K. (2003)  Plant Cell, 12, 2856-65

We are making other movies for teaching purpose as well and will release them whenever we finish.

Thanks, Takato
Takato Imaizumi Ph.D.
Associate Professor
Department of Biology
University of Washington
24 Kincaid Hall, Box 351800
Seattle, WA 98195-1800
Tel (office): 206-543-8709
Tel (lab): 206-221-0325
Web: http://faculty.washington.edu/takato/

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