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[Plant-education] <no subject>

David Alan Walker d.a.walker at sheffield.ac.uk
Sat Jul 8 11:21:50 EST 2006

photosynthesis lab for no-nmajors biology (gross at georgian.edu)

RE:     ³I did run into a leaf-disk lab with bicarbonate that we
might try².

 Why look further than the Leaf-Disc Electrode?

 [DELIEU T, DA WALKER 1981 Polarographic measurement of photosynthetic O2
evolution by leaf discs. New Phytol 89: 165-175]

  To paraphrase the words of the immortal Mandy Rice-Davis
<http://en.wikipedia.org/wiki/Mandy_Rice-Davies > ŒI would say that,
wouldn't I ?¹ but what subsequently became increasingly sophisticated as,
for example, in

 [ WALKER DA, CB OSMOND 1986 Measurement of photosynthesis in vivo with a
leaf disc electrode: correlations between light dependence of steady-state
photosynthetic O2 evolution and chlorophyll a fluorescence transients. Proc
R Soc B 227: 267-280]

 was first conceived as a simple teaching device and is still  commercially
available (in its simplest as well as its much more sophisticated automated
forms) from Hanatech Instruments
<http://www.hansatech-instruments.com/form_1.htm>. Deeply exhaled air (near
enough 5% CO2) suffices as a source of carbon dioxide and you can get good
kinetic measurements with a combination of digital readout, stopwatch,
neutral density filters etc.



>From David Walker, FRS., Emeritus Professor of Photosynthesis, University of
Sheffield, UK.

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