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Measuring Growth of Anabaena

Carl Pike carl.pike at fandm.edu
Wed Mar 3 07:59:22 EST 2004

You might do a simple assay for chlorophyll as a proxy for cell 
number.  Extract the cells in acetone or methanol, spin down debris, 
and measure spectrophotometrically.  In combination with Warwick's 
suggestion, you could construct a calibration curve of cell number 
(hemocytometer) vs. chlorophyll - but that is probably more than you 
need, although it might be informative for the students to see the 

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