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tissue culture question

Schmid, Katherine kschmid at butler.edu
Fri Feb 14 15:18:30 EST 2003


We actually have been having pretty good luck with Carolina's carrot 
callus initiation medium, which we follow 6 weeks later with carrot 
shoot initiation medium.  It is necessary to add 30 g/l sugar plus agar 
or agargel to these media.  As suggested by someone earlier, carrots 
that still have the tops are most successful.  We aim for the vascular 
cambium region when taking explants.  The recurring problem has been 
contamination, but at least part of the class succeeds.  Also, there's 
no time to root the shoots before the semester is over....  Some 
students get light green fluffy callus, and some get the anthocyanins.  
I've not been able to discern a pattern.

_____________________________
Dr. Katherine M. Schmid
Dept. of Biological Sciences
Butler University
4600 Sunset Ave.
Indianapolis IN  46208
317-940-9956
kschmid at butler.edu
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