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Post-Doc available on Stomata

James Morison morisj at essex.ac.uk
Wed Aug 12 13:07:16 EST 1998

Post Doctoral Research Fellow
3 years, UK BBSRC funded

[[[[[ Evaluating the contributions of guard and mesophyll cell
photosynthesis to the regulation of stomatal movements ]]]]]

**Introduction & Project aims
Understanding stomatal physiology and function is critical to predicting
the response of plants to environmental changes and to stress. There are
two particularly important questions that remain unanswered: 1) What is the
function of the nearly ubiquitous chloroplasts within guard cells? and 2)
What role does photosynthesis within mesophyll cells play in controlling
stomatal conductance? This study will address both of these questions, in
situ, through the application of high resolution imaging of chlorophyll
fluorescence and reflected light to examine the relationship between
photosynthesis and stomata under a range of environmental stresses,
particularly temperature, light and drought.

**Proposed work
We have developed a unique system (Oxborough & Baker, 1997, Plant, Cell &
Environ. 20:1473-83) which is able to image reflected light and chlorophyll
fluorescence, within a few seconds of each other, at spatial resolutions
that are high enough to measure stomatal apertures (from the reflected
light images) and to determine values of meaningful fluorescence parameters
from individual guard cells and surrounding mesophyll cells (see Oxborough
& Baker, 1997 for examples). This allows for temporal analyses of the
relationships between stomatal movements and the efficiency and regulation
of photosynthesis within guard and mesophyll cells, in intact leaves, in
controlled environmental conditions. This promises a considerably advance
on previous chlorophyll fluorescence work with guard cells which used
either epidermal peels, or variegated leaves in order to separate mesophyll
from guard cell. The work will be able to link together gas exchange with

We require a plant physiologist who has recently completed a PhD in a
stomatal physiology, or a relevant area of photosynthesis research. The
ideal applicant would have considerable expertise with gas exchange
techniques, and some familiarity with chlorophyll fluorescence. A general
background and interest in environmental plant biology is necessary. The
post is available from mid-October, 1998.

The appointee will join a large research group in environmental biology,
with several staff with long interests in photosynthesis and stomatal
function. In addition to the imaging system described above, the Dept. is
well equipped with  state-of-the-art equipment for plant gas exchange
studies, chlorophyll fluorescence, and controlled environment cabinets.

**Contact & other details

see  details and web links on  <http://www.essex.ac.uk/bcs/jobs/stomata.html>

Dr James Morison 	tel. +44 (0)1206 - 873327
email: morisj at essex.ac.uk

Prof Neil Baker		tel. +44 (0)1206 - 873319
email :baken at essex.ac.uk

fax: +44 (0)1206- 873416

Dr James I.L. Morison,
Department of Biological Sciences,
John Tabor Laboratories,
University of Essex,
Wivenhoe Park,
Colchester    CO4 3SQ   UK
tel +44 (0)1206 873327      fax +44 (0)1206 873416
email:morisj at essex.ac.uk

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