A post-doctoral research fellowship is available to study
protein-subunit interactions in the Photosystem l reaction center.
The investigations include:
Elucidation of the domain of the FX cluster on the core heterodimer.
The structure of the binding site for the PsaC subunit.
The mechanism of the PsaC subunit/cofactor core interaction.
The de novo design and synthesis of structures relevant to
Photosystem 1 structure and electron transfer mechanisms.
The position is funded by the National Science Foundation and
can be filled immediately. Candidates should be qualified in
biochemistry/molecular biology and preferably have expertise
in protein chemistry and molecular cloning. Alternatively,
applicants from the physical/chemical sciences will be considered
if they have training in optical spectroscopy/signal processing/computer
technology etc and are interested in learning protein biochemistry
and molecular methods.
Recent references relevant to the project are:
Rodday, S., M., S-S Jun and Biggins, J. (1993) Interaction of the
FAFB-containing subunit with the Photosystem l core heterodimer.
Photosyn. Research 36, 1-9.
Rodday, S., M., Schulz, R., McIntosh, L. and Biggins, J. (1994)
Structure-function studies on the interaction of PsaC with the
core heterodimer. The site-directed change R561E in PsaB destabilizes
the subunit interaction in Synechocystis sp. PCC 6803. Photosyn.
Research 42, 185-190.
Rodday, S., M., Webber, A., N., Bingham, S., E. and Biggins, J. (1995)
Evidence that the PsaB interhelical loop in the FX domain in
Photosystem l interacts with the subunit PsaC. Site-directed
changes in PsaB destabilizes the subunit interaction in Chlamydomonas
reinhardtii. Biochemistry 34, 6328-6334.
Candidates interested in the position should contact
Dr. John Biggins
Department of Molecular and Cell Biology & Biochemistry
Providence, RI 02912 Tel: 401 863 2540
Fax: 401 863 1201
E Mail: BIGGINS at BROWNVM.BROWN.EDU