I have recently measured some spectra of thylakoids suspension
ascorbate reduced- ferricyanide oxidized. As I know cytochrome f
should have a maximum at 554 nm, but my results are slightly different.
The maximum is shifted about 3-4 nm to higher wavelenghts.
Is there a reasonable explanation of this fact?
I would appreciate any suggestion (maybe this are some ascorbate
reducable thylakoid components visible in that region which I do not know).
Thanks in advance
Rafal Ziobro, ubziobro at jetta.if.uj.edu.pl
ul. Armii Krajowej 81/9, 30-150 Krakow, tel. 37-20-77