Without knowing more specifics on what exactly
you are doing and what sort of cultures you are
trying to obtain it is difficult to advise you on how
to proceed. It is also not clear what you mean by
contamination - the Locke's-Egg medium and
Robinson's medium are intended for xenic culture
so there must be bacteria present for the amoebae
to grow. I may be able to help but I need more information!
In case you have not already done so I suggest you
read the pages on Entamoeba cultivation on the
Entamoeba Homepage site:
C. Graham Clark, Ph.D.
Department of Infectious and Tropical Diseases,
London School of Hygiene and Tropical Medicine,
Keppel St., London WC1E 7HT
Entamoeba Homepage: www.lshtm.ac.uk/mp/bcu/enta/home.htm