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Long PCr primers

Wellcome Trust, Nairobi. wellcome at USERS.AFRICAONLINE.CO.KE
Sat Nov 8 08:40:24 EST 1997

> Dear Colleagues,
> If there any risk of amplifying a fragment DNA with primer which are 50 to 60 pb. Actually, I want to introduce a enzyme restriction site in these primers so that I can easily  detected them in agore or Acrylamide gel. Therefore I do need longer primers. But I wandering about doing a PCR such long oligonucleotides.
> My regards,

Wellcome Trust Research Labs, 
PO Box 43640,
Nairobi, Kenya.
Tel 254 (2) 725390, Fax 254 (2) 711673
E-mail: wellcome at users.africaonline.co.ke

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