Postdoctoral position is available in the laboratory of Dr. Dan S. Ray in
the Molecular Biology Institute at UCLA for studies of the structure and
replication of kinetoplast DNA. Current research areas of investigation
include the role of H1 histone-like proteins in the condensation and
organization of kinetoplast DNA and the protein composition and structure
of kinetoplast-associated replisomes. Strong biochemistry background and
publications in major journals required. Experience in protein
purification and characterization necessary. For further information on
research areas in the laboratory see recent publications below:
Pasion, S., Hines, J., Aebersold, R. and Ray, D.S. (1992) Molecular
cloning and expression of the gene encoding the kinetoplast-associated
type II DNA topoisomerase of Crithidia fasciculata. Molec. and Biol.
Parasit. 50, 57-69.
Ray, D.S., Hines, J. and Anderson, M. (1992) Kinetoplast-associated DNA
topoisomerase in Crithidia fasciculata: Crosslinking of mitochondrial
topoisomerase II to both minicircles and maxicircles in cells treated with
the topoisomerase inhibitor VP16. Nucl. Acids Res. 20, 3353-3356.
Brown, G.W., Fisher, P., Hines, J.C. and Ray, D.S. (1994) Isolation of the
genes encoding the 51 kilodalton and 28 kilodalton subunits of Crithidia
fasciculata replication protein A. Molec. Biochem. Parasitol. , 63,
Pasion, S.G., Brown, G.W., Brown, L.M. and Ray, D.S. (1994) Periodic
expression of nuclear and mitochondrial DNA replication genes during the
trypanosomatid cell cycle. J. Cell Sci., 107, 3515-3520.
Ray, D.S. and Hines, J.C. (1995) Disruption of the Crithidia fasciculata
RNH1 gene results in the loss of two active forms of ribonuclease H. Nucl.
Acids Res. 23, 2526-2530.
Xu, C.W., Hines, J.C., Engel, M.L., Russell, D.G. and Ray, D.S. (1996)
Nucleus-encoded histone H1-like Proteins are associated with kinetoplast
DNA in the trypanosomatid Crithidia fasciculata. Mol. Cell. Biol. 16,
For further information, contact
Dr. Dan S. Ray
Molecular Biology Institute
Los Angeles, CA 90095-1570
danray at mbi.ucla.edu