I'm having a difficult time fixing crypto oocysts to microscope slides. I have used a standard procedure of air drying
purified oocysts, fixing with 95% ethanol, washing with PBS, followed by application of the antibody per IFA.
Apparently all the oocysts are washed away.
Question: what procedures do others use to directly fix crypto to microscope slides?
Jim Wick
E-mail: wick at cris.com
Phone: 414-871-7199
FAX: 414-871-1273
Snail Mail: 5520 W. Burleigh St.
Milwaukee, WI 53210
"Don't precipitate more than you can resuspend"
