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Crypto: safe handling

Mon Jun 12 18:07:27 EST 1995

To:            parasite at net.bio.net
From:          wick at cris.com
Subject:       Crypto: safe handling
Date:          29 May 1995 15:10:29 GMT

My lab is just beginning to handle viable Cryptosporidium oocysts. I would like to generate a standard procedure for the safe handling 
of crypto, methods for decontaminating surfaces and how to handle the generated waste. Any input from those of you experienced 
with this organism would be greately appreciated. Please note, this method must be in place before I can begin any of my 

Jim Wick		

E-Mail	wick at cris.com	 	"Don't precipitate more than 
Mail:	5520 W. Burleigh St.	  you can resuspend."
	Milwaukee, WI 52310
Phone	414-871-7199		
FAX	414-871-1273	 	

Decontaminate spills with 4% ammonia (it makes your eyes stream and your 
nose run but it kills oocysts).  Autoclave all waste.

Try to use heat inactivated (80 C for 10 min) instaed of live oocysts 
where ever possible.

Have fun.

Graham Vesey
Australian Environmental Flow Cytometry Group                                        
School of Biological Sciences,     .-.--:_:\
Macquarie University,            _/         \
Sydney,                         :   AEFCG    |
Australia NSW 2109.             \_          /
Tel- 612 850 8150                 '-''''\__/
Fax- 612 850 8174                        V  

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