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Tue Feb 21 12:57:15 EST 1995

Re trichinellosis in Georgia swine.  We had only limited opportunity 
to examine seropositive swine at slaughter.  We obtained diaphrams 
from a number (don't recall the exact figure, but between 20 and 40) 
of either seropositive swine or swine from seropositive herds.  By 
digesting the entire diaphragm, we recovered larvae from only 3 sows. 
 They harbored, 1, 1, and 2 in the entire diaphragm.  What does this 
mean?  I am uncertain.  Possibly other larvae were calcified in the 
tissue and were not revealed by the digestion procedure, or possibly 
the swine had a wildlife species of Trichinella.
My graduate student, Dr. Kelly Mann, isolated Trichinella T5 from a 
bobcat here in Georgia and identified it using DNA technics.  Mr. 
ChaoQun Yao, another student, has examined the infectivity of T5 
versus that of T1 (spiralis).  In swine he found that administration 
of T5 caused seroconversion at 5 weeks after infection in contrast to 
3 wai for spiralis.  But, at necropsy 8 wai, T5 could NOT be 
recovered from any of the tissues digested.  There were also 
differences in infectivity and fecundity in various laboratory hosts. 
 Of interest, examination of several immunodominant antigens by 
recombinant DNA technics reveal few (less than 5% difference) in the 
antigen sequences.  Swine infected with spiralis cross-react with T5.
Katherine Prestwood

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