Can I have a bit more details?
How do you cut the slices?
What kind of stimulating electrode do you use?
How do you choose the intensity of your stimulus?
On Aug 13, 2:06 am, Min-Yu Sun <msun... from student.ucr.edu> wrote:
>> I meet a problem for using the LTD protocol to induce LTD. I am using a
> PP-LFS protocol (1Hz, 900 paired-pulses; pulse interval:50 ms, pulse
> duration: 0.1 ms) to induce LTD on acute hippocampal slices from wild-type
> mice (aged from P17-P23). My amplifier is Axopatch 200B, and the software I
> use is pClamp 10.2. All the experiments are done in room temperature (22-25
> C). The problem is, I can't induce LTD reliably. Sometimes I can induce LTD
> for about 15-20% at 45-60 min after PP-LFS (rise slope of fEPSP is 80-85%
> compared to baseline level), but sometimes I can't induce LTD at all even if
> I use exactly the same protocol. The rise slope of fEPSP went back to around
> 95-100% (compared to baseline) at 30 min after PP-LFS. I was just wondering
> if there is any experimental condition I might not pay attention to, which
> could extremely affect LTD induction. Does anyone have any thought about
> this? I 'd very much appreciate for any possible suggestions!
>> Thanks a lot!
>> Amy (Min-Yu)