This wont be much help, but I know a very experienced Ca2+ imager, who
bought some SBFI, and couldn't get it to work at all, so you're not
the only person who is having trouble with this stuff.
On Sep 25, 1:00 pm, Yishan Sun <sys... from gmail.com> wrote:
>> I am trying to use the sodium indicator SBFI to measure neuronal sodium
> influx in response to depolarization. I found several references, but none
> of them precisely described a method for loading of SBFI (in the form of AM
> ester) to cultures of dissociated neurons. If you have used this technique
> in your own studies, may I learn from your experience?
>> Have a nice weekend!