Hi all,
We have just acquired a head stage from A-M systems attached to a 1600
Neuroprobe amplifier for extracellular single recording in
anesthetized rats. Has any of you used it?
We still have a lot of noise in the signal, and I am not sure of how
to use it: what should I use as a ground? should I connect a ground
wire to the headstage or directly to the amplifier? any advice to
obtain better recordings?
I'd qould appreciate any comment
Ana
.
El 16/06/2010, a las 19:04, neur-sci-request from oat.bio.indiana.edu
escribió:
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>>> Today's Topics:
>> 1. Re: Different reversal potential or just poor space-clamp?
> (jonesmat)
> 2. Last call for applications ----- NWG-Course: Analysis and
> Models in Neurophysiology, Freiburg, Germany (Janina Kirsch)
>>> ----------------------------------------------------------------------
>> Message: 1
> Date: Tue, 15 Jun 2010 13:56:41 -0700 (PDT)
> From: jonesmat <jonesmat from physiology.wisc.edu>
> Subject: [Neuroscience] Re: Different reversal potential or just poor
> space-clamp?
> To: neur-sci from net.bio.net> Message-ID:
> <944dff09-c0a5-4309-b94a-f7b1edc7f6dd from c10g2000yqi.googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>> On Jun 5, 12:25 am, Bill <connelly.b... from gmail.com> wrote:
>> Hi,
>> So I'm using whole-cell voltage clamp to record from a cell that has
>> two classes of inhibitory inputs. One to the soma, and one (largely)
>> to the dendrites, which I can activate independently. I/V plots show
>> me that the apparent reversal potential of the dendritic input is
>> 10mV
>> lower than the somatic input. This could be due to the fact that a)
>> there is active Cl- homeostatis in the dendrites, which means that
>> the
>> reversal potential truly is lower there, or b) I have poor space
>> clamp, and hence I have to drive the somatic voltage below the actual
>> reversal potential to get the potential at the dendrite to the
>> reversal potential. (or I suppose both). There is plenty of evidence
>> that I don't have proper voltage clamp of the dendritic events
>> (slower
>> rise and decay time).
>>>> Is there any way to figure out whether this effect is solely due to
>> cable filtering of my command voltage?
>>> Hi Bill,
>> There is a way to estimate the electrotonic attenuation between your
> headstage and the synapse, though it's a little involved. It relies on
> giving voltage jumps at a series of times leading up to, and
> following, stimulation of the synapse.
>> The method is thoroughly described here, with real data and
> simulations to test its accuracy:
>> Estimating the time course of the excitatory synaptic conductance in
> neocortical pyramidal cells using a novel voltage jump method.
> Häusser M, Roth A.
> J Neurosci. 1997 Oct 15;17(20):7606-25.
>> Cheers,
>> Matt
>>>>>>>> ------------------------------
>> Message: 2
> Date: Wed, 16 Jun 2010 13:30:50 +0200
> From: "Janina Kirsch" <kirsch from bcf.uni-freiburg.de>
> Subject: [Neuroscience] Last call for applications ----- NWG-Course:
> Analysis and Models in Neurophysiology, Freiburg, Germany
> To: "'AlifeAnnounce'" <alife-announce from lists.idyll.org>, "'CompNeuro'"
> <comp-neuro from neuroinf.org>, "'CompNeuro'" <comp-neuro from neuroinf.org>,
> "'Connectionists'" <connectionists from cs.cmu.edu>, "'CVNET'"
> <cvnet from mail.ewind.com>, "'Visionlist'"
> <visionlist from visionscience.com>,
> <Neur-sci from magpie.bio.indiana.edu>
> Message-ID: <001401cb0d47$5fc842b0$1f58c810$@uni-freiburg.de>
>> %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
> %% NWG-Course: Analysis and Models in Neurophysiology %%
> %% October 10-15, 2010 %%
> %% Application deadline: June 30, 2010 %%
> %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
>> Aim of the course
> The course is intended to provide advanced Diploma/Masters and PhD
> students,
> as well as young researchers from the neurosciences with approaches
> for the
> analysis of electrophysiological data and the theoretical concepts
> behind
> them.
>http://www.bcf.uni-freiburg.de/events/conferences/101010-nwgcourse>> The course includes various topics such as
> • Neuron models and spike train statistics
> • Point processes and correlation measures
> • Systems and signals
> • Local field potentials and synaptic plasticity
> • FIND: New tools and developments
>> The course will consist of lectures in the morning and and matching
> exercises using Matlab and Mathematica. Experience with these software
> packages will be helpful but is not required for registration. The
> participants should have a basic understanding of scientific
> programming.
> This course is designated especially for advanced diploma/master-
> students
> and PhD-students (preferentially in their first year).
>> Organisation and teaching:
> • Dr. Stefan Rotter, Bernstein Center Freiburg, University of Freiburg
> • Dr. Sonja Gruen, RIKEN Brain Science Institute
> • Dr. Ulrich Egert, Biomicrotechnology, Department of Microsystems
> Engineering, Faculty of Engineering, University of Freiburg
> • Dr. Ad Aertsen, Neurobiology & Biophysics, Faculty of Biology,
> University
> of Freiburg,
>> Contact:
> Dr. Janina Kirsch,
> Bernstein Center Freiburg
> Germany
> Tel: +49 761 203 9575
> Fax: +49 761 203 9559
> Email: nwg-course from bcf.uni-freiburg.de>> Application
> Please apply by sending an email containing your CV and a letter of
> motivation to
>nwg-course from bcf.uni-freiburg.de>> The course is limited to 20 participants.
> Course fees:
> NWG members: 50 Euro
> others: 125 Euro
>> Course venue:
> Bernstein Center Freiburg, Computerlab (ground floor), Hansastr. 9a,
> 79104
> Freiburg, Germany
>>>>> ------------------------------
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