I'm trying to selectively stimulate a nucleus but there are axons that
pass through the nucleus. I used minimal stimulation on the assumption
that cell bodies have a lower threshold for extracellular stimulation
than axons, but this did not satisfy the reviewers. So I want to try
on-cell stimulation. My question is, how much current do you think I
will need to pass through the on-cell electrode (so I can figure out
what piece of equipment I can use), and how will I know if I am
succeeding in generating an action potential? I should be able to see
an on-cell spike?