Dear Colleagues,
I am wondering if anyone can share with me there experiences using a
peristaltic pump for perfusions (e.g., what specific equipment, tubing size,
flow rate settings). Currently, I am using a Masterflex L/S economy drive
pump for our perfusions and have noticed some inconsistent results from time
to time which I believe might be related to our setup for perfusion. Any
help would be appreciated.
Lastly, does anyone have experience flash freezing tissue using liquid N2
cooled isopentane? If so, could you share with me your specific setup. I
have explored some alternatives (e.g., dry ice, isopentante/dry ice
slurries) and my experience suggests that liquid N2/isopentante produces the
best results (but not as perfect as I would like), so I would like to get a
little more detail of what most people are using. I have far more
experience sectioning on a vibrating microtome and I am not use to seeing
freeze fracture artifacts (i.e., swiss chesse effect), so I tend to--maybe
unrealistically--have a fairly high standard for tissue quality.
Thanks in advance,
Neil