Hello,
I am working on perfusion and fixation of rat brains for the purpose of
Fluoro-Jade staining after the brain has been sectioned with a cryostat.
I am having problems with the brain sections looking like "Swiss
cheese". Has anyone else had this problem? What can I do about this
problem? I am using 4% paraformaldehyde in PBS. Any info/hints will be
greatly appreciated.
Thank you,
Mary Brownson, Ph.D.
School of Pharmacy
University of Wyoming
(307)766-6748
