>>>> I am working on perfusion and fixation of rat brains for the purpose of
> Fluoro-Jade staining after the brain has been sectioned with a cryostat.
> I am having problems with the brain sections looking like "Swiss
> cheese". Has anyone else had this problem? What can I do about this
> problem? I am using 4% paraformaldehyde in PBS. Any info/hints will be
> greatly appreciated.
>>>> Thank you,
>>>> Mary Brownson, Ph.D.
>> School of Pharmacy
After perfusing the brains, I normally sit them in 4% paraformaldehyde for a
day, and then put them into a 30% sucrose solution overnight for a second
day. This helps to cryoprotect the brains and stop them disintigrating when
you cut them.