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[Neuroscience] Re: Cerebellar slices, advice?

Bill via neur-sci%40net.bio.net (by connelly.bill from gmail.com)
Tue Mar 20 17:00:50 EST 2007


Thanks for the tip!


On Mar 21, 5:09 am, "NL Cerminara, Physiology"
<N.Cermin... from bristol.ac.uk> wrote:
> Hi
>
> I'm not a cerebellar slice person but I know that some people prefer to use
> older animals because morphological and functional maturation of rat mossy
> fiber-granule cell synapses appears to occur much later, with ontogenesis
> complete by P60 see Hamori and Somogyi 1983; Wall and Usowicz 1998).
>
> Nadia
>
> . --On 19 March 2007 15:33 -0700 Bill <connelly.b... from gmail.com> wrote:
>
> > Hi,
>
> > I need to do some cerebellar slices. I'm trying to figure out the best
> > age band and the best orientation of the slice.
>
> > I see some people using very young animals (P10-20) for cerebellar
> > slices for no apparent reason. Though others use months old animals.
> > Apart from the general, "younger animals make nicer slices" rule of
> > brain slices, is there any specific cut off I should be aware of? (I'm
> > after granule cells)
>
> > Also, what the the best plane of section to cut the slices in. The one
> > time I tried making cerebellar slices, it reminded me of trying to
> > shave a cauliflower, little tiny bits fell of, rather than big proper
> > slices. I would imagine you would either glue it to the stage of the
> > vibrotome via the sagital mid-line, or along the ventral surface of
> > the brain stem.
>
> > Any tips would be appreciated,
>
> > Thanks in advance.
>
> > _______________________________________________
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> > Neur-... from net.bio.net
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