Thanks for the help Matt. I sent Dempster an email, and he informed me
that WinEDR is the program I want to use. I see now that it has an
inbuilt program for doing all of this. Not that I've gotten it to work
yet (all I get in the PS is massive power at low frequencies,
decreasing exponentially), but I will see what I can get any more
sense out of your advice (I wonder if my sampling/filtering is what is
messing me up).
On Jun 23, 10:49 am, jonesmat <jones... from physiology.wisc.edu> wrote:
> Hi Bill,
>> This analysis basically involves computing the power spectrum of chuck
> of data, then fitting that to a Lorentzian function. To take the PS
> you use the FFT, which I think that Dempster's software should be able
> to do. Sometimes people use tricks to smooth the data and get better
> fits, but this is not absolutely essential if you have nice long
> stretches of clean data. One trick is to repeat the FFT on multiple
> segments, then average the FFTs.
>> Anyway, try looking at this old paper for an example:
>> Segal M, Barker JL.
> Rat hippocampal neurons in culture: properties of GABA-activated Cl-
> ion conductance.
> J Neurophysiol. 1984 Mar;51(3):500-15.
> PMID: 6321695 [PubMed - indexed for MEDLINE]
>> BTW, usually you want to do this TWICE: once with the channel active,
> then once with it blocked. Then you subtract those two PSs from each
> other to isolate the spectrum of the channel of interest.
>> Cheers,
>> Matt
