Hey,
Today I cut thalamocortical slices (from 5 week of mice) along the
lines of the Agmon and Connors 1991 paper. I had to cut them thinner
than they suggested (300uM). I can't even seem to get a cortical field
potential when I stimulate in the thalamus. I'm guessing this means
I'm selecting slices that are too far caudal/rostral or possibley one
of many other reasons. The slices themselves are viable (hippocampal
field potential, cortical cells are normal from the intracellular
point of view).
Anyone got any ideas?
Thanks.
