I am a PhD student and I am starting with the electrophysiology. We are
trying to record neuronal activity in rats during urethane anesthesia
but we are having lots of problems with this. I am first anesthetizing
with isofluorane and then 15% urethane in distilled water I.P. at 1.5 to
1.8 g/kg. Usually it takes a lot of time for them to loss reflexes and
sometimes they die before we could start recording (and also they use to
die very early). I also use local topic lidocaine in pressure points.
Any advise? What dose should I use?