[Neuroscience] siRNA transfection of primary neurons

O'Hare, Michael via neur-sci%40net.bio.net (by Michael.OHare At nrc-cnrc.gc.ca)
Tue Nov 14 12:40:11 EST 2006

Has anybody managed to successfully transfect primary cultures of
cerebellar granule neurons with short double stranded siRNA?  If so,
what transfection reagent did you use and how much RNA and transfection
reagent needs to be combined to achieve optimal transfection?  Any
additional details that could be provided would also be greatly
appreciated.  Thank you.



Michael J. O'Hare, Ph.D.

Tel: (613) 993-4294 | Fax: (613) 941-4475 | email: michael.ohare At nrc.ca


Institute for Biological Sciences

National Research Council of Canada

1200 Montreal Road, Building M-54

Ottawa, Ontario K1A 0R6, Canada



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