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Staining of neurons: biocytine vs. lucifer yellow?

Darwin darwin at beagle.edu
Thu May 12 13:45:26 EST 2005

I guess you've seen lots of examples of the labelling already, but here's a 
web site with a nice confocal pic of a lucifer yellow filled granule neuron 
from the rat.  You can even follow the axon for quite some distance in their 
paper I think.


<tehgabriel at web.de> wrote in message 
news:1115894014.112677.152940 at f14g2000cwb.googlegroups.com...
> Hi!
> So far i have no experience with staining of cells e.g. cortical
> neurons. For a new set of experiments we thought it might be useful to
> start with this in order to discriminate certain morphological criteria
> (some dendritic projections, general shape of dendritic tree etc.). It
> seems that the most common methods for staining are via biocytine or
> via lucifer yellow. What are the advantages/disadvantages of these two
> compounds?
> So far as i know, lucifer yellow is very easy to use, requires no
> histochemical preparation but does not stain fine dendritic structures.
> Biocytine, on the other hand, allows a finer resolution but requires
> some histochemics after the recording.
> What do you think/is your experience?
> Every tip or comment is appreciated.
> Cheers,
> Thomas

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