IUBio

[Neuroscience] Re: Nystatin for perforated patches

bilz0r at hotmail.com bilz0r at hotmail.com
Thu Jul 7 04:26:50 EST 2005


Unfortunately, I wouldnt be able to do that, because I'm working in the
slice, blind.

Christian Wilms wrote:
> SJM Guzman <jose.guzman at medizin-uni-leipzig.de> wrote:
>
> > I think is a good idea to monitor the contact (with the series
> > resistance), but anyhow... is not true that if you have a "whole-cell"
> > configuration the diffusion of the antibiotic would immedialtely lead to
> > the death of cell ?
>
> Danke für die Blumen :-)
>
> To be honest, I have never done perforated patch myself, but got very
> close at an early point of my doctoral work - hence my PI gave me a lot
> of advice, which I have never gotten down to trying myself. As far as I
> understood, the problem with monitoring ther series resistance would be
> that membrane breakdown would only have to be fairly small in order die
> allow washout, and hince would not have to make a significant difference
> in the series resistance.
>
> I did however get to enjoy the advantages of the bridge-mode when doing
> long-term measurements ... ah ... the comfort of it is amazing. I can
> only recommend it :-)
> 
> bye, Christian



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