I am interested if any one in this group have experience using Timm staining
for mossy fiber growth. If so, could you please forward me the protocol
that you typically use. I am aware that this stain is quite finicky, thus I
would only appreciate comments and responses from those who have actual
experience using this technique in the laboratory setting.
Also I am interested in obtaining TOAD-64 antibodies. Has anyone here used
these antibodies using ICC techniques? If so, I would like to know your
opinions for a tentative development of a ICC protocol. I am specifically
interested in using this as an additional marker for postmitotic, immature
neurons labelling. (i.e. in conjunction with BrdU immunostaining). Any
suggestion and advice are extremely appreciated.