The following Online PCR primer design tools are available at
Primo Pro --- Standard PCR, reduces primer dimer and random primering.
Primo Multiplex --- Multipe PCR in one tube. Reduces dimers and random
Primo Degenerate --- Degenerate primers based on single protein, or
multiple alignments of proteins or nucleotides
Primo Diverse --- Single pair of primers to amplify regions that are
most diverse in a gene family
Primo Unique --- Multiple primer pairs, each uniquely amplify one gene
in a family
Primo MSP --- Methylation-specific PCR primers
Comments and suggestions are especially welcome.