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A suite of online PCR primer design tools

Chang Zhu czhu at changbioscience.com
Thu Oct 24 14:01:11 EST 2002

The following Online PCR primer design tools are available at


Primo Pro --- Standard PCR, reduces primer dimer and random primering.
Batch mode.

Primo Multiplex --- Multipe PCR in one tube. Reduces dimers and random

Primo Degenerate --- Degenerate primers based on single protein, or
multiple alignments of proteins or nucleotides

Primo Diverse --- Single pair of primers to amplify regions that are
most diverse in a gene family

Primo Unique --- Multiple primer pairs, each uniquely amplify one gene
in a family

Primo MSP --- Methylation-specific PCR primers

Comments and suggestions are especially welcome.



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