"Mike" <m-thurston at northwestern.edu> wrote in message news:<Hd2Y8.2468$931.1172682 at newssrv26.news.prodigy.com>...
> Does anyone have any reccommendations for sectioning a whole rabbit brain at
> 50 microns on a cryostat. I'm having trouble keeping the "peices" together.
> I didn't know if there was a certain embedding material that is best for a
> brain of this size.
>> thanks
>> Mike
Hi Mike!
50 microns is not bad for a piece of brain whit the size of 5-6
sq.inches.
I will recomend you to play first whit the temperature.Set it low
about -25,-30 C,It depends also what type of cryostat you are using
.For big sectioning is also good to use vibrating microtom.
