PrimerBase is a small and practical database for storing, searching,
and designing of oligo-nucleotides. Convenient functions of PrimerBase
includes:
1. Database: Stores primer information in an searchable database.
2. Searching: Searches primers using target sequences in both
sense/antisense directions.
3. Primer Design: Finds primers with optimal annealing temperature and
GC content. Check for primer-primer dimers.
Give it a try by downloading at
http://www.geocities.com/virtualab/primerbase/primerbase.html