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How to avoid cell cluster in neuron culture?

joe joe at wer.wer
Sun Oct 28 00:54:37 EST 2001


lay em down on a "stickier" substrate. what did you pre-coat your dishes
with? Often cells find sticking to their neighbors better than to the
dish.....unless of course, it is coated with "optimal" substrate.

lv rui wrote in message
<20011013191614.63439.qmail at web10804.mail.yahoo.com>...
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>I have cultured some primary neurons from P5 rat
>hippocampus, with Neural basal containing B27, bFGF,
>glutamaxin as culture medium. On 3DIV, I found many
>cluster cellualr islands. I think the density of cells
>might be too high (400/mm2). Is anyone has good ideas
>of avoiding cells cluster?
>
>Jackl
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