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Help with DRG cell culture

Nick Jacobsen nickjacobsen at yahoo.co.uk
Tue Oct 9 07:14:44 EST 2001


Hi,

Can anyone help me with dissociated DRG cell culture. i prepare the
culture and grow for 24hours in DMEM w/FCS/glutamine/NGF. On day 2 i
change to neurobasal medium w/glutamine/B27/NGF and antimitotics FdU
and uridine. After two days i change to neurobasal w/glu/B27/NGF. By
day7 the cultures are dead and at this point id rather they were
living,

can anyone help me out here ?

thanks very much in advance

Nick




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