Hi,
Can anyone help me with dissociated DRG cell culture. i prepare the
culture and grow for 24hours in DMEM w/FCS/glutamine/NGF. On day 2 i
change to neurobasal medium w/glutamine/B27/NGF and antimitotics FdU
and uridine. After two days i change to neurobasal w/glu/B27/NGF. By
day7 the cultures are dead and at this point id rather they were
living,
can anyone help me out here ?
thanks very much in advance
Nick