* Richard Norman <rsnorman at mediaone.net> wrote:
>> Your question is not quite clear : do you talk about human striatum, or
>> other species (cat's ?) ; of an adult , I presume ?
>> Are you looking for the volume per cell (cell volume) or looking for the
>> total mass of the corpus striatum (total number of cells in that
>> structure?)
>> The volume as such ? or the mass (in weight) ?
I am looking for the (average) volume of a single neuron cell in human
striatum.
>I am also curious to know how you plan to use the volume of cells
>to calculate concentrations. What data do you already have and what
>are you trying to do? Apparently you have measured the quantity
>(grams or moles) of some material taken from a brain sample and
>want to calculate an appropriate concentration. But what indication
>do you have that the material you measured was specifically
>restricted to the intracellular volume of striatum neurons, as
>opposed to glia or trapped in the extracellular space? Those
>factors are critical in determining what volume to use.
We didn't use brain but cell cultures to do our investigations. To get
the amount of compound uptaken by the cells, the cells could be lysed
with a certain amount of lysing buffer in which the compound
concentration could be determined. Unfortunately, without knowing the
cell volume it is impossible to obtain the concentration in cell.
But for this reason, it es really only the volume inside the cells
relevant for the calculation.
Thanks a lot,
Gunter