Henk Veldman <H.Veldman-2 at neuro.azu.nl> wrote:
> Angie schreef:
>> > Does anyone know of a robust and fairly clean antibody to use for
> > histochemistry that localizes to the cytoplasm of glia, preferably
> > astrocytes, both activated and non activated?
> >
> > Thanks for any help!
> >
>> Almost any producer of commercial antibodies will have one or more
> preparation in its catalog against GFAP (glial fibrillary acidic
> protein), both polyclonal and monoclonal. Try DAKO, Sigma, Chemicon,
> well . . . any.
> We are using the DAKO polyclonal antibody on sections of fixed rat brain
> and on cultured cells to identify and locate astrocytes, much to our
> satisfaction.
>> Henk
Yes, we are waiting on a new batch of GFAP, however it was our
understaning that in tissue it stains primarily activated astrocytes. (Am
I wrong about that?) We are trying to find an antibody that will stain
both activated and non-activated astrocytes in tissue sections. We have a
reaction product from another histochemical stain that we are trying to
co-localize. The first reaction is staining nuclei throughout the cortex,
and we are trying to find out if the nuclei are from neurons or
astrocytes. We have tried S100-beta, but as it results in nuclear
staining as well, it's a bit problematic.
Thanks,
Angie
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