"Dallas" <dallas.grasby at adelaide.edu.au> wrote in message
news:396433C9.6F5BC3E9 at adelaide.edu.au...
> Could someone using the Sigma anti-TH antibody (T-1299) please give me a
> hint as to the dilution appropriate for labelling cells in fixed tissue
> sections - I am yet to use the antibody in sections (rat brain paraffin
> embedded).
Everyone tells me that you always have to "empirically determine" the
appropriate dilution when you get a new antibody. Try a couple ranges like
1:500, 1:1000, 1:5000, or call sigma and ask them what they recommend for
fixed tissue.
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