Hi everybody
I am trying to augment cAMP dependent protein kinase (PKA) activity in a
neuron, in order to reveal it's impact on certain ionotrophic events in
an acutely isolated dentate granule cell, during voltage clamp
recording. For this purpose I think to include PKA catalytic subunit
into my recording pipette. however, I don't know what should be right
(closed to physiologically relevant) concentration of the protein. media
etc. Also, I need to inhibit PKA. Which inhibitors and what
concentrations are recommended?
I will highly appreciate if someone will share his/her experience and
suggestions on this matter.
