Does anyone know wether rodent pituitary intermediate lobe (IL) also
produces growth hormone?
I am now trying to surgically isolate clean IL from anterior lobe tissues.
There I am using RT-PCR to check my IL tissues not including AL by detecting
GH mRNA. Even though I do LCM (laser captured microdissection), which is
supposed to be very precise, I always see GH mRNA. I am wondering whether
there is a little bit of somatotrophs in IL too, or IL has leaky expression
of GH transcripts.
Thank you in advance... And a Happy New Year.
Mari Shinohara, Ph.D.
Joslin Diabetes Center
Boston, MA 02215