Would a vital stain like methylene blue work?
It should stain the nerve well enough so that you can dissect it out of the
capsule.
Soon Hyouk Lee wrote in message <7u2qm8$u2j$1 at merrimack.Dartmouth.EDU>...
>Hello!
>>I'm a student working on a project in studying regenerated contacts after
>transplantation between two different species of crayfish. However, I have
>been unable in determining a method to tag the nerve so that it is visible
>after the transplantation is complete. After the nerve has been
>transplanted, the recipient forms a protective capsule around the
>transplant, and the proliferation of connective tissue around the site of
>injury which makes it virtually impossible to find the nerve. I have been
>only been able to record spontaneous activity in the muscle cells after the
>new contacts have been made, but would like to stimulate the transplanted
>nerve.
>>Is there anyone that can give suggestions on how to tag the nerve so that
it
>can be seen after the transplantation?
>>thank you,
>>Soon Hyouk Lee
>>Soon.Hyouk.Lee at dartmouth.edu
