IUBio

Transplant Dyes for nerves in superficial flexor muscle system?

Soon Hyouk Lee Soon.Hyouk.Lee at dartmouth.edu
Wed Oct 13 15:39:33 EST 1999


Hello!

I'm a student working on a project in studying regenerated contacts after
transplantation between two different species of crayfish.  However, I have
been  unable in determining a method to tag the nerve so that it is visible
after the transplantation is complete.  After the nerve has been
transplanted, the recipient forms a protective capsule around the
transplant, and the proliferation of connective tissue around the site of
injury which makes it virtually impossible to find the nerve.  I have been
only been able to record spontaneous activity in the muscle cells after the
new contacts have been made, but would like to stimulate the transplanted
nerve.

Is there anyone that can give suggestions on how to tag the nerve so that it
can be seen after the transplantation?

thank you,

Soon Hyouk Lee

Soon.Hyouk.Lee at dartmouth.edu



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